TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000333681
  • Gene Name:BCL2
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qRT-PCR
  • mRNA Pathway:apoptotic signaling pathway
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB06774
  • Drug Name:Capsaicin
  • Drug Method:down-regulated the gene expression the gene expression

Disease basic infommation

  • Disease:acute lymphoblastic leukemia
  • Tissue:cell line (CCRF-CEM)

Other information

  • Title:Capsaicin induced apoptosis and gene expression dysregulation of human acute lymphoblastic leukemia CCRF-CEM cells
  • Journal:J buon
  • Published:2014
  • PubMed ID:24659662
  • Abstract:Purpose:Capsaicin, an ingredient of red chili pepper, has possible tumorigenicity/genotoxicity properties. We aimed to determine the effects of capsaicin on the proliferation and gene expression profiles of acute lymphoblastic leukemia (ALL) CCRF-CEM cell line. Methods:Cell viability and IC50 dose was determined by WST cytotoxicity assay. qRT-PCR, immunohistochemical staining and western blot methods were used to determine target genes＇ expression levels. Apoptosis was evaluated by measuring the caspase-3 activity. Results:Capsaicin inhibited the proliferation of CCRFCEM cells in a dose-dependent manner. Increased mRNA expressions of caspase gene family members, activated caspase-3 and decreased mRNA and protein expression of BCL-2 gene indicated apoptotic response to capsaicin. Moreover capsaicin treatment suppressed significantly the expression of the key cell signaling pathways of KRAS, AKT, GAB2, PTPN11, BRAF, INPP5D, MAPK7. Conclusion:Capsaicin induces apoptosis in CCRF-CEM cells and this response is associated with downregulation of cell signaling pathways.
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