TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000263923
  • Gene Name:KDR
  • mRNA database:Ensembl
  • mRNA expression :up-regulated
  • mRNA Method:qRT-PCR
  • mRNA Pathway:NA
  • Evidence:predicted
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:NA
  • Drug Method:NA

Disease basic infommation

  • Disease:non-small cell lung cancer
  • Tissue:NSCLC cancer cell line Calu-1,H358,H460,H292,H1650,A549,H1299

Other information

  • Title:miR-497 suppresses malignant phenotype in non-small cell lung cancer via targeting KDR.
  • Journal:Oncology reports
  • Published:2019
  • PubMed ID:31115562
  • Abstract:The aim of the present study was to evaluate the expression of microRNA-497 (miR-497) in non-small cell lung cancer (NSCLC) tissues and cell lines, and to investigate possible mechanisms associated with its regulatory role on cell behaviors. The expression level of miR-497 was evaluated in 15 cases of NSCLC tissues and 8 adjacent normal tissues, and in 8 NSCLC cell lines, including H1975, A549, H358, H1650, H460, Calu-1, H1299 and H292, by reverse transcription-quantitative polymerase chain reaction. Effects of miR-497 overexpression on cell proliferation, invasion, apoptosis and radiosensitivity were examined with a Cell Counting Kit-8 assay, Matrigel assay, flow cytometry and a clone formation assay in vitro, respectively, and in an in vivo ectopic tumor nude mice model. A dual luciferase reporter assay was employed for interaction between miR-497 and its target gene kinase insert domain receptor (KDR). A significantly decreased level of miR-497 was determined in NSCLC tissues, compared with adjacent normal tissues, and Calu-1 and H1975 exhibited the lowest miR-497 expression among the 8 NSCLC cell lines. miR-497 overexpression could inhibit cell proliferation and invasion, promote cancer cell apoptosis and decrease cell clone formation following radiation treatment in vitro, and decrease tumor growth in vivo. Furthermore, a dual luciferase reporter assay revealed that KDR as the target gene for miR-497. It was demonstrated that miR-497 was downregulated in NSCLC specimens. Additionally, miR-497 directly targeted and downregulated KDR expression, and inhibited malignant behaviors of NSCLC cells. These data indicated that miR-497 could serve as a tumor suppressor gene involved in NSCLC pathogenesis.
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