TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000590099
  • Gene Name:BECN1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qRT-PCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB00877
  • Drug Name:Sirolimus
  • Drug Method:down-regulated the gene expression

Disease basic infommation

  • Disease:catagma
  • Tissue:tissues

Other information

  • Title:Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy
  • Journal:Journal of Orthopaedic Surgery And Research
  • Published:2023
  • PubMed ID:36814286
  • Abstract:Background:While autophagy is essential for stem cells＇ self-renewal and differentiation, its effect on bone marrow mesenchymal stem cells (BMSCs) remains unclear. This study aimed to investigate the interaction between autophagy and osteogenic differentiation using rapamycin (RAPA), a classical autophagy agonist with osteo-regulatory effects. Methods:Rat BMSC＇s autophagy was analyzed after osteoinduction (0, 7, 14, and 21 d) by western blotting, immunofluorescence, and real-time quantitative polymerase chain reaction (RT-qPCR). In addition, we evaluated osteogenic differentiation using alizarin red staining, alkaline phosphatase assays, and RT-qPCR/Western blotting quantification of bone sialoprotein, type 1 collagen, alkaline phosphatase, osteopontin, and Runt-related transcription factor 2 mRNA and protein levels. Results:The BMSC＇s basal autophagy level gradually decreased during osteogenic differentiation with a decrease in BECN1 level and the lipidated (LC3-II) to unlipidated (LC3-I) microtubule-associated protein 1 light chain 3 ratio and an increase in the expression of selective autophagic target p62. In contrast, it increased with increasing RAPA concentration. Furthermore, while 2 nM RAPA promoted BMSC osteogenic differentiation on days 7 and 14, 5 nM RAPA inhibited osteogenesis on days 14 and 21. Inhibition of autophagy by the inhibitor 3-methyladenine could impair RAPA＇s osteogenesis-enhancing effect on BMSCs. Conclusions:The BMSC＇s basal autophagy level decreased over time during osteogenic differentiation. However, an appropriate RAPA concentration promoted BMSC osteogenic differentiation via autophagy activation.
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