TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000216117
  • Gene Name:HMOX1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:p38-MAPK
  • Evidence:predicted
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:NA
  • Drug Method:NA

Disease basic infommation

  • Disease:oesophageal squamous carcinoma
  • Tissue:Eca109 human oesophageal squamous carcinoma cells

Other information

  • Title:Upregulation of HO-1 is accompanied by activation of p38MAPK and mTOR in human oesophageal squamous carcinoma cells.
  • Journal:Cell biology international
  • Published:2013
  • PubMed ID:23412940
  • Abstract:Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinoma cells. Real-time PCR and Western blots were used to determine the expression levels of HO-1, p38MAPK, p-p38MAPK and p-mTOR. MTT assays were used to measure proliferation, FACS for cell cycle events and Annexin V staining for apoptosis. Proliferation of Eca109 cells was inhibited and apoptosis was induced in the presence of p38MAPK inhibitor (SB203580) and mTOR inhibitor (Rapamycin, RAPA). HO-1 expression was downregulated in cells treated with SB203580 and RAPA. HO-1 overexpression inhibited apoptosis and induced G2/M arrest in SB203580 and RAPA-treated cells. HO-1 expression was upregulated in the presence of ethanol, and was accompanied by activation of p38MAPK and mTOR. However, ethanol-treated cells exposed to HO-1 inhibitor showed no effect on p38MAPK and mTOR activation. The data suggest that ethanol-induced upregulation of HO-1 in oesophageal squamous cell carcinoma is accompanied by the activation of the p38MAPK and mTOR pathways.
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