TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000344347
  • Gene Name:XBP1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qPCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB00112
  • Drug Name:Bevacizumab
  • Drug Method:down-regulated the gene expression

Disease basic infommation

  • Disease:non-small cell lung cancer
  • Tissue:cell line A549

Other information

  • Title:Bevacizumab induces A549 cell apoptosis through the mechanism of endoplasmic reticulum stress in vitro
  • Journal:International Journal of Clinical And Experimental Pathology
  • Published:2015
  • PubMed ID:26191230
  • Abstract:Aims:To observe the effect of bevacizumab on human A549 cells and explore its mechanism. Methods:After different concentrations (0 μM, 1 μM, 5 μM, 25 μM) of bevacizumab treating in A549 cells, CCK8 assay detect the impact of bevacizumab on A549 cell proliferation and flow cytometry determine the effect of bevacizumab on human A549 cells apoptosis. Real-time PCR and Western blotting detect the changing expression of the target gene (CHOP, caspase-4, IRE1, XBP-1) on mRNA and Protein level. Results:Treatment with bevacizumab for 24-hr have induced cell death in a does-dependent manner dramatically (P<0.05). In terms of the mRNA level, expression of XBP-1 has increased obviously in each group (1 μM, 5 μM, 25 μM) (P<0.01); the expression of CHOP (25 μM) and caspase-4 (5 μM) have increased slightly (P<0.05). In terms of the protein level, the expression of CHOP has increased obviously in each group (1 μM, 5 μM, 25 μM) when compared with the control group (0 μM) (P<0.05). As for caspase-4 (5 μM, 25 μM), the expression have increased slightly when compared with the control group (0 μM) (P<0.05). Conclusion:Bevacizumab can induce A549 cell apoptosis through the mechanism of endoplasmic reticulum stress.
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