TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000395915
  • Gene Name:AURKA
  • mRNA database:Ensembl
  • mRNA expression :up-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:NA
  • Evidence:predicted
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:NA
  • Drug Method:NA

Disease basic infommation

  • Disease:bladder cancer
  • Tissue:Human bladder cell lines 5637, T24, J82, RT4 81 and urothelial immortalized cell SV-HUC-1

Other information

  • Title:MicroRNA-124-3p affects proliferation, migration and apoptosis of bladder cancer cells through targeting AURKA
  • Journal:Cancer biomarkers : section A of Disease markers
  • Published:2017
  • PubMed ID:28269755
  • Abstract:Objective:The aim of this study was to establish the relationship between miR-124-3p and Aurora A kinase (AURKA) in bladder cancer (BC). Methods:The expressions of miR-124-3p and AURKA in BC tissues and cell lines were detected using RT-PCR and western blot. BC cells were transfected with miR-124-3p mimics and AURKA siRNA. After this cell proliferation, migration, cell cycle and apoptosis were measured using CCK-8, colony formation assay, wound healing assay and cytometry tests. The relationship between miR-124-3p and AURKA was confirmed with luciferase reporter assay. Mice xenograft models were constructed to examine the effects of AURKA on BC in vivo. Results:MiR-124-3p expression was significantly down-regulated in BC tissues and cell lines, while AURKA was significantly up-regulated compared to normal samples. MiR-124-3p targeted AURKA and decreased its expression. Transfection of miR-124-3p mimics and AURKA siRNA was shown to down-regulate BC cell proliferation and migration as well as induce cell apoptosis. As suggested by xenograft models, the inhibition of AURKA can effectively suppress tumor growth. Conclusion:MiR-124-3p has significant impact on proliferation, migration and apoptosis of BC cells by targeting AURKA.
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