TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000345358
  • Gene Name:BAX
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:Ganodermanontriol
  • Drug Method:down-regulated the gene expression

Disease basic infommation

  • Disease:hepatocellular carcinoma
  • Tissue:patients tissues

Other information

  • Title:Ganodermanontriol inhibits expression of special AT rich sequence binding protein 1 gene in human hepatocellular carcinoma
  • Journal:Journal of Cancer Research And Therapeutics
  • Published:2018
  • PubMed ID:30539830
  • Abstract:Aims:This study explored mainly on the mechanism how ganodermanontriol affects the expression of SATB1 and inhibits proliferation of tumor on human hepatoma cell line HepG2. Settings and design:The cancer cells were treated with ganodermanontriol. The status of the cells was detected by different methods. The mechanism was checked by various methods. Materials and methods:In HepG2 cancer cells treated with various concentrations of ganodermanontriol, the cell proliferation of was detected by MTT assay, cell apoptosis was analyzed by flow cytometry; the mRNA of SATB1, Bcl-2, Bax were detected by reverse transcription-polymerase chain reaction (RT-PCR) and the protein level of SATB1, Bcl-2, Bax, and caspase 3 were analyzed by Western blot. Statistical analysis used:Data are presented as the mean ± standard deviation. The data were analyzed using SPSS 18.0 software (SPSS, Inc., Chicago, IL, USA) and GraphPad Prism software (GraphPad Software, Inc., La Jolla, CA, USA). A one-way analysis of variance test was used to compare the differences among groups. Results:This study showed that ganodermanontriol could significantly reduce the expression level of SATB1. Conclusion:Therefore, downregulate the cascade effect caused by the expression level of Bcl-2 in HCC HepG2 cells.
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