TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000216281
  • Gene Name:HSP90
  • mRNA database:Ensembl
  • mRNA expression :up-regulated
  • mRNA Method:qRT-PCR
  • mRNA Pathway:NA
  • Evidence:predicted
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:NA
  • Drug Method:NA

Disease basic infommation

  • Disease:melanoma
  • Tissue:Human melanoma cell line A375 cells

Other information

  • Title:Columbamine suppresses proliferation and invasion of melanoma cell A375 via HSP90-mediated STAT3 activation.
  • Journal:J Recept Signal Transduct Res.
  • Published:2021
  • PubMed ID:32669028
  • Abstract:Purpose:The goal of this study is to explore the effects of columbamine in melanoma cells and the signaling pathway involved. Methods:Human melanoma cell line A375 cells were used in this study. Cell proliferative ability was detected by MTT assay and clone formation assay. Cell migration and invasion were measured by wound healing assay and transwell assay, respectively. Protein expression was examined by Western blotting. Results:Columbamine reduced cell proliferative ability and the number of clone spots in A375 cells. Western blotting results demonstrated that expression of cleaved caspase 3, an activated cell death protease, was upregulated by 20 and 50 μM of columbamine. Wound healing results showed that the scratch width was wider in cell treated with 20 and 50 μM of columbamine than that in cell treated with 0 and 10 μM of columbamine. Phosphorylation of STAT3 and expression of HSP90 was also repressed by columbamine in a concentration-dependent manner. Overexpression of HSP90 attenuated the inhibition of cell proliferation, migration and invasion induced by columbamine. Conclusion:Columbamine inhibited melanoma cell proliferation, migration, and invasion in A375 cells through inactivation of STAT3, which is mediated by HSP90.
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