TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000296533
  • Gene Name:NPY1R
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:Northern blot
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB00755 (NUTR00051, APRD00362)
  • Drug Name:All-trans retinoic acid (ATRA)
  • Drug Method:down regulated the gene expression

Disease basic infommation

  • Disease:neuroblastoma tumour
  • Tissue:cell line (SK-N-MC )

Other information

  • Title:Retinoic acid is a negative regulator of the neuropeptide Y/peptide YY Y1 receptor gene in SK-N-MC cells
  • Journal:J Neurochem
  • Published:1997
  • PubMed ID:8978705
  • Abstract:To identify effectors of neuropeptide Y Y1 receptor gene expression predicted by putative regulatory elements in its 5＇ flanking region, we examined the effects of several transcriptionally active agents on Y1 receptor mRNA levels and 125I-peptide YY binding capacity in SK-N-MC cells. Phorbol ester caused a rapid, transient 2.6-fold increase in Y1R mRNA levels. However, all trans-retinoic acid caused a rapid, sustained decrease in Y1 receptor mRNA levels (to 25% of control). Cycloheximide pretreatment did not attenuate the maximal inhibitory effect of retinoic acid, but it prolonged the time to achieve maximal efficacy. The retinoic acid effect was secondary to both a significant decrease in Y1 receptor mRNA stability and a decreased Y1 receptor gene transcription rate. Y1 receptor binding capacity was also significantly decreased after retinoic acid treatment (368 +/- 25 vs. 496 +/- 28 fmol/mg of protein for control). These data support a role for retinoic acid as an important agent regulating Y1 receptor gene expression and mediating Y1 receptor down-regulation.
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