TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000397062
  • Gene Name:NFE2L2
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:NRF2 signaling pathway
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB12025
  • Drug Name:Triptolide
  • Drug Method:down-regulated the gene expression the gene expression

Disease basic infommation

  • Disease:myeloid leukemia
  • Tissue:cell lines (HL60/A,K562/G)

Other information

  • Title:Triptolide, a Chinese herbal extract, enhances drug sensitivity of resistant myeloid leukemia cell lines through downregulation of HIF-1alpha and Nrf2
  • Journal:Pharmacogenomics
  • Published:2013
  • PubMed ID:23930677
  • Abstract:Aim:To explore whether triptolide (TPL) can enhance drug sensitivity of resistant myeloid leukemia cell lines through downregulation of HIF-1α and Nrf2. Materials & methods:HL60/A and K562/G cells were subjected to different treatments and thereafter an methyl thiazole tetrazolium bromide assay, flow cytometry, western blot and real-time PCR were used to determine IC??, apoptotic status and expression of Nrf2, HIF-1α and their target genes. Results:Doxorubicin- or imatinib-induced apoptosis was enhanced when anticancer agents were used in combination with TPL. When combined with TPL, both doxorubicin and imatinib downregulate Nrf2 and HIF-1α expression at protein and mRNA levels. Genes downstream of Nrf2, for example, NQO1, GSR and HO-1, as well as target genes of HIF-1α, for example, BNIP3, VEGF and CAIX are also downregulated at the mRNA level. Conclusion:TPL is able to enhance drug sensitivity of resistant myeloid leukemia cell lines through downregulation of HIF-1α and Nrf2.
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