TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000267163
  • Gene Name:RB1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qRT-PCR
  • mRNA Pathway:NA
  • Evidence:predicted
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:NA
  • Drug Method:NA

Disease basic infommation

  • Disease:gastric cancer
  • Tissue:Human GC cell lines AGS, SGC-7901, NCI-N87, GES-1, MKN-45, BGC-823

Other information

  • Title:miR-215 promotes cell migration and invasion of gastric cancer by targeting Retinoblastoma tumor suppressor gene 1.
  • Journal:Pathology, research and practice
  • Published:2017
  • PubMed ID:28689850
  • Abstract:Background:Gastric cancer (GC) is one of the most common malignant tumor and has high mortality worldwide. microRNAs (miRNAs) play critical roles in carcinogenesis. Previous studied showed that miR-215 was involved in tumorigenesis and progression. This study was designed to clarify the biological function of miR-215 in GC. Methods:qRT-PCR was used to detect the miR-215 expression in GC tissues and 6 human GC cell lines (AGS, SGC-7901, NCI-N87, GES-1, MKN-45 and BGC-823) as well. Transwell assay was used to investigate the biological function of miR-215 in GC. Luciferase reporter assay was used to confirm its effect on the regulation of the target gene Retinoblastoma tumor suppressor gene 1 (RB1). Results:miR-215 was frequently up-regulated in GC tissues compared to adjacent non-tumor tissues and GC cell lines. miR-215 expression level was correlated with the progression of tumor invasion and tumor-node-metastasis (TNM) stage. Over-expression miR-215 in GC cell lines promoted cell migration and invasion. Besides, miR-215 could down-regulate the expression of RB1 in vitro via directly binding to its 3＇-untranslated region (UTR), while the expression of RB1 would suppress the miR-215-indueced GC cell migration and invasion. Conclusions:miR-215 promoted cell migration and invasion of gastric cancer by directly targeting RB1.
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