TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000413366
  • Gene Name:PRKCA
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qPCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:NA
  • Drug Name:GO6983
  • Drug Method:down-regulated the gene expression

Disease basic infommation

  • Disease:colitis
  • Tissue:tissues

Other information

  • Title:Protein kinase D1 mediates synergistic MMP-4 expression induced by TNF-α and bradykinin in human colonic myofibroblasts
  • Journal:Biochemical And Biophysical Research Communications
  • Published:2011
  • PubMed ID:21867693
  • Abstract:Stromal myofibroblasts regulate extracellular matrix components through the secretion of matrix metalloproteinases such as MMP-3. Both myofibroblasts and MMP-3 have been implicated in colonic inflammation and cancer but the regulatory signaling mechanism(s) are unknown. Exposure of the human colonic myofibroblast cell line 18Co to TNF-α and bradykinin induced synergistic MMP-3 mRNA and protein expression, which were blocked by the preferential PKC inhibitors GF109203X and Go6983 and by the MEK inhibitor U0126. Transfection with siRNA targeting PKD1, a known downstream target of both bradykinin and PKC, completely inhibited MMP-3 mRNA and protein expression. Our results imply that TNF-α and bradykinin amplify MMP-3 expression at a transcriptional level through a signaling cascade involving PKC, PKD1, and MEK. PKD1 plays a critical role in the expression of MMP-3 in human colonic myofibroblasts, and may contribute to the pathophysiology underlying colitis-associated cancer.
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