TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000264657
  • Gene Name:STAT3
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:JAK-STAT3 signalin pathway
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB11672
  • Drug Name:Curcumin
  • Drug Method:inhibit JAK kinase activity to inhibit the STAT3 phosphorylation

Disease basic infommation

  • Disease:skin cancer
  • Tissue:cell lines (A431)

Other information

  • Title:Inhibitory effect of curcumin on invasion of skin squamous cell carcinoma A431 cells
  • Journal:Asian Pac J Cancer Prev
  • Published:2015
  • PubMed ID:25854367
  • Abstract:Objective:To investigate the regulatory effect of curcumin on expression of signal transducer and activator of transcription 3 (STAT3) in skin squamous cell carcinoma tissues as well as possible mechanisms of curcumin in prevention and treatment of skin squamous cell carcinoma. Materials and methods:Highly invasive A431 cells were treated with curcumin at various doses .The cytotoxic effects of treatment with 5, 10, 15, 20, 25, 30, 35, 40 and 50 umol/L curcumin for 24, 48 and 72 hours on A431 cells were measured by MTT assay. The invasion capacity of cells treated with 5, 10 and 15 umol/L curcumin was measured by Transwell test, while adhesive ability was assessed by cell adhesion assay. The effects of 5,10 and 15 umol/L curcumin on expression levels of STAT3 were determined by Western blotting and on transcription levels of STAT3 mRNA by RT-PCR. Results:Treatment with curcumin at a doses of more than 15 umol/L for more than 24 hour inhibited the growth of A431 cells in a time-and dose-dependent fashion (p<0.001). The doses of 15 umol/L and less for 24 hours showed no significant cytotoxic effects on the cells, survival rates being more than 85%.The invasion and adhesive abilities decreased gradually with the increasing curcumin concentration, 15 umol/L exerting the strongest inhibitory effects (p<0.05). Curcumin showed significant dose-dependent inhibitory effects on the transcription level of STAT3 mRNA (p<0.05). Conclusions:Curcumin may reduce the invasive ability of A431 cells by inhibiting the activation of STAT3 signal pathway and expression of STAT3 as a target gene in the pathway.
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