TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000374867
  • Gene Name:NRP1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qRT-PCR
  • mRNA Pathway:SP1 signlaing pathway
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB03568 (EXPT00774)
  • Drug Name:Butyric Acid
  • Drug Method:down-regulated the gene expression the gene expression

Disease basic infommation

  • Disease:colon cancer
  • Tissue:cell lines (Caco-2, HCT116,HT29)

Other information

  • Title:Butyrate suppresses expression of neuropilin I in colorectal cell lines through inhibition of Sp1 transactivation
  • Journal:Mol Cancer
  • Published:2010
  • PubMed ID:20950431
  • Abstract:Background:Neuropilin is a transmembrane receptor for vascular endothelial growth factor (VEGF) and is expressed in normal endothelial cells and upregulated in cancer cells. Neuropilin-1 (NRP-1) has been shown to promote tumour cell migration and survival in colon cancer in response to VEGF binding. The expression profiles of neuropilins, associated co-receptors and known ligands have been mapped in three colorectal cell lines: Caco-2, HCT116 & HT29. We have previously shown that butyrate, a naturally occurring histone deacetylase inhibitor (HDACi) produced by fermentation of fibre in the colon, causes apoptosis of colon cancer cell lines. Results:Here we demonstrate that butyrate down-regulates NRP-1 and VEGF at the mRNA and protein level in colorectal cancer cell lines. NRP-1 is a known transcriptional target of Sp1, whose activity is regulated by acetylation. NRP-1 down-regulation by butyrate was associated with decreased binding affinity of Sp1 for canonical Sp-binding sites in the NRP-1 promoter. siRNA-mediated knock-down of Sp1 implied that Sp1 may have strong DNA binding activity but weak transactivation potential. Conclusion:The downregulation of the key apoptotic and angiogenesis regulator NRP-1 by butyrate suggests a novel contributory mechanism to the chemopreventive effect of dietary fibre.
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