TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000271751
  • Gene Name:EAG1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB00136 (NUTR00003, APRD00246, DB05314)
  • Drug Name:Calcitriol
  • Drug Method:down-regulated the gene expression the gene expression

Disease basic infommation

  • Disease:cervical cancer
  • Tissue:cell lines (SiHa, HeLa, PC-3,MCF7)

Other information

  • Title:Calcitriol down-regulates human ether a go-go 1 potassium channel expression in cervical cancer cells
  • Journal:Anticancer Res
  • Published:2010
  • PubMed ID:20682996
  • Abstract:Background/aim:Human ether à-go-go-1 (EAG1) potassium channels are promising anticancer targets. Calcitriol has antitumoural properties. This study investigated EAG1 regulation by calcitriol in normal and cancer cells. Materials and methods:Cancer cell lines from cervix, prostate, mammary gland, and normal placenta trophoblasts were cultured. Calcitriol was determined by HPLC. Gene and protein expression were assessed by real-time RT-PCR and western blot analysis, respectively. Calcitriol-synthesising enzyme CYP27B1 or vitamin D receptor (VDR), were transfected in cervical cancer SiHa cells. Cell proliferation was assayed with XTT. Results:Calcitriol decreased EAG1 mRNA in all cell types, and EAG1 protein and proliferation in SiHa cells. VDR antagonist ZK-159222 prevented the calcitriol effect on EAG1 mRNA. CYP27B1-transfected cells produced more calcitriol and less EAG1 mRNA. EAG1 mRNA was more potently inhibited by calcitriol in VDR-transfected cells. Conclusion:EAG1 is a calcitriol target in normal and cancer cells and calcitriol is a potential therapy for cervical cancer.
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