TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000231751
  • Gene Name:LTF
  • mRNA database:Ensembl
  • mRNA expression :up-regulated
  • mRNA Method:RT-PCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB01262
  • Drug Name:5-Aza-2-deoxycytidine (5-Aza-CdR)
  • Drug Method:demethylating the promoter to induce the mRNA expression

Disease basic infommation

  • Disease:acute t lymphoblastic leukemia
  • Tissue:cell line (molt-4)

Other information

  • Title:Effects of decitabine against acute T lymphoblastic leukemia cell line Molt4
  • Journal:Zhonghua Xue Ye Xue Za Zhi
  • Published:2015
  • PubMed ID:25854468
  • Abstract:Objective:To explore the effects and possible mechanisms of decitabine on Molt4 in vitro. Methods:Effects of decitabine on cells proliferation were detected by using CCK-8, the apoptosis by Annexin V-FITC, cell cycles by propidium iodide-FACS. Discrepancy genes were screened by RNA-seq technique. The CpG methylation of lactoferrin (LTF) gene in Molt4 cells were identified by Bisulfite sequencing PCR (BSP). The expression of LTF mRNA in Molt4 by RT-PCR and LTF protein expression were analyzed by Western blot. Results:Decitabine effectively inhibited proliferation and induced apoptosis for Molt4 cells by an time- and dose-dependent manners. Cell cycles were arrested at the G?/G? phase. The promoter methylation degree of LTF gene in Molt4 cells was 72.3% before decitabine treatment and decreased to 45.0% after treatment with 0.50 μmol/L decitabine for 72 h. After the reduction of methylation, expression of its mRNA and protein increased, meanwhile caspase 3 and caspase 9 protein expression levels increased. Conclusion:The demethylating drug decitabine can induce apoptosis, detain cell cycle at phase G?/G?, inhibit proliferation and up-regulate LTF gene expression in Molt4 cells. LTF may become a new target for acute T lymphoblastic leukemia.
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