TargetRx Atlas

MRNA basic information

  • mRNA ID:ENST00000447510
  • Gene Name:PROM1
  • mRNA database:Ensembl
  • mRNA expression :down-regulated
  • mRNA Method:qPCR
  • mRNA Pathway:NA
  • Evidence:validated
  • (mRNA-drug)

Drug basic information

  • Drug ID:DB06816
  • Drug Name:Pyrvinium
  • Drug Method:down-regulated the gene expression

Disease basic infommation

  • Disease:lung cancer
  • Tissue:patients tissues

Other information

  • Title:β-catenin inhibitors suppress cells proliferation and promote cells apoptosis in PC9 lung cancer stem cells
  • Journal:International Journal of Clinical And Experimental Pathology
  • Published:2017
  • PubMed ID:31966561
  • Abstract:This study aimed to investigate the effect of β-catenin inhibitors on cells proliferation and apoptosis in lung cancer stem cells (LCSCs). Drug-resistance PC9 cells were induced by escalation of cisplatin repeated treatment, and then PC9 LCSCs were constructed by Sphere Formation methods. Membrane expression of OCT4, SOX2, CD44, CD133 and β-Catenin were detected by Immunofluorescent staining, and mRNA of CSCs marker genes and Wnt/β-Catenin target genes were determined by qPCR assay. PC9 LCSCs were nurtured for 5 days (Day 5) and then β-catenin inhibitor pyrvinium pamoate (PP) with IC50concentration (0.221 碌M) and ICG-100 with IC50concentration (2.620 碌M) were added and cultured for another 2 days (Day 7), respectively. CCK8 and AV/PI assays were performed to detect cells proliferation and apoptosis. We successfully constructed PC9 LCSCs and observed that OCT4, SOX2, CD44, CD133 and β-Catenin expressed on all cells, and stem-cell marker genes as well as Wnt/β-Catenin signaling pathway genes mRNA were all elevated in PC9 LCSCs compared to PC9 parent cells. Cells proliferation by CCK8 assay was decreased while apoptosis rate by AV/PI assay was increased in PP treatment group compared with control, C-Caspase 3 and Bcl-2 protein expression also supported the apoptosis results. Most of the stem-cell marker genes and Wnt/β-Catenin signaling pathway genes mRNAs were decreased accordingly. ICG-001 also inhibited cells proliferation while induced cells apoptosis in PC9 LCSCs. In conclusion, β-Catenin inhibitors suppressed the proliferation and promoted the apoptosis of LCSCs, which shed light on a new potential target for lung cancer treatment.
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